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Chunmei Yang, Xiaojing Ma, Congcong Zhang
This research explores how using a birthing ball alongside free positioning enhances the childbirth experience and outcomes for first-time mothers. A randomized controlled trial was conducted involving 200 nulliparous women, equally divided into an intervention group utilizing both birthing balls and free positions, and a control group following conventional supine delivery methods. Findings revealed that the intervention group experienced a notably shorter first stage of labor, averaging 5.8 hours compared to the control group's 7.2 hours. Additionally, the incidence of perineal injuries was reduced to 25% versus 41% in the control group. Pain levels, measured by VAS scores, were significantly lower at 6.2 points compared to 7.5 points in the control group. Maternal satisfaction rates also improved, reaching 82% in the intervention group against 65% in the control group. These results demonstrate that integrating birthing balls with free positioning effectively shortens labor duration, decreases perineal trauma, alleviates pain, and boosts maternal satisfaction, highlighting its clinical applicability. The study offers valuable scientific evidence and practical insights for optimizing delivery experiences among nulliparous women.
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Qiongwen Xu, Bo Jin
The histone modification H3K4me3 plays an important role in regulating gene expression and displays extensive reprogramming on the genome during critical stages of mammalian embryonic development. In this study, we collected porcine oocytes and early fertilized embryos at various stages, and used immunofluorescence staining as well as low-cell-volume chromosome immunoprecipitation sequencing to investigate the changing pattern of key histone modification H3K4me3 during the development of early fertilized porcine embryos. The methylation levels of porcine germ cells with in vivo embryos at different developmental stages were detected by RRBS method. The HOT of embryonic stem cells (H1) to middle endoderm cells (ME), neural precursor cells (NPC), trophoblast cells (TBL), and mesenchymal stem cells (MSC) was analyzed by in-depth investigation of the dynamic law of change of the genetic traits (HOT) during the development and differentiation of embryonic development in the critical period. The results showed that the failure of H3K4me3 broad to narrow peak conversion on the promoter of ZGA-related genes at the 4-Cell stage in the knockdown group was detected by immunofluorescence staining as well as ChIP-seq. These results suggest that H3K4me3 affects early embryonic development mainly by influencing the occurrence of ZGA and thus the development of the early embryo, further suggesting the role of genetic traits in the critical period of porcine embryonic development.
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Mingming Song, Yunxi Yang, Bingwei Sun
Through the clinical analysis of wound edge samples of patients with chronic infectious wounds, we found that there were a good deal of acute and chronic inflammatory cells infiltrating around the wound edge of chronic wounds, most of which were neutrophils. Prior investigations have demonstrated the apoptosis of neutrophils is significantly delayed in the state of infection, and a good deal of neutrophils with delayed apoptosis gather around the wound. Whether it will affect the wound healing has not been fully clarified. In this study, we used PIM kinase inhibitor to promote the apoptosis of neutrophils and thus promote the wound healing by making infected wounds in rats. After LPS stimulation of the rat's back wound, the neutrophils at the edge of the wound infiltrated a lot, the expression profile of CD31 decreased significantly, and the expression of PIM kinase and inflammatory factors at the edge of the wound increased significantly; After the use of PIM kinase inhibitor, the expression of CD31 at the wound margin increased significantly, and the expression of PIM kinase and inflammatory factors at the wound margin decreased significantly. LPS stimulation of neutrophil delayed apoptosis can inhibit the proliferation of HMEC1; The use of PIM447 can promote the apoptosis of neutrophils and thus the proliferation of HMEC1. The mechanism study found that LPS can promote the expression of Bcl2 and downregulate the transcriptional profile of BAX and Caspase3 via the Bcl2/Caspase3 signaling pathway, thereby facilitating the expression of PIM kinase. Our data shows that: LPS can delay neutrophil apoptosis in infectious wounds caused by Gram-negative bacteria, resulting in a large amount of neutrophil infiltration at the wound margin, which inhibits the proliferation of endothelial cells. The mechanism by which LPS inhibits neutrophil apoptosis may involve promoting the expression of neutrophil PIM kinase and regulating the Bcl2/Caspase3 pathway.
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Yuqian Zhao, Jing An, Huaqing Liu, Huiqin Ding
Objective This study aimed to explore the effect of structured psychological nursing intervention based on psychological capital theory on the psychological state of patients in the Intensive Care Unit (ICU) and the mental health of their families. Methods A randomized controlled trial design was adopted. A total of 120 conscious patients with an expected length of stay in the ICU of ≥7 days and 240 primary caregiving family members admitted to the ICU of a Grade A tertiary hospital from January to December 2023 were selected as the research subjects. They were randomly divided into an intervention group and a control group, with 60 patients and 120 family members in each group. The control group received conventional psychological nursing, while the intervention group implemented a structured psychological nursing intervention program based on psychological capital theory in addition to conventional nursing. Evidence-based nursing techniques such as goal-setting technology, cognitive restructuring method, positive suggestion therapy, and stress coping training were adopted. Each intervention lasted for 30-45 minutes, was conducted once every other day, and a total of 6 interventions were carried out. The intervention period was from 48 hours after admission to the ICU until before transfer out of the ICU. Results After the intervention, the score of the Psychological Capital Questionnaire of patients in the intervention group was significantly higher than that in the control group (42.85±6.12 vs 36.24±5.78, P<0.001), and the scores of the Hamilton Anxiety Scale and Hamilton Depression Scale were significantly lower (Anxiety: 8.45±3.21 vs 12.67±4.15, P<0.001; Depression: 7.92±2.89 vs 11.43±3.67, P<0.001). The psychological capital score of family members in the intervention group was significantly higher than that in the control group (45.76±7.23 vs 38.92±6.45, P<0.001), and the score of the Zarit Caregiver Burden Interview was significantly lower (28.34±5.67 vs 35.28±6.92, P<0.001). Conclusion The structured psychological nursing intervention based on psychological capital theory can effectively improve the mental health status of ICU patients and their families, and enhance their psychological adaptability and coping efficacy.
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Yifei Wang, Tao Lv, Xu Zhang, Hao tian
Background and Aim: Acupuncture promoting bone mass has long been applied to the treatment of osteoporosis. The aim of this study was to evaluate the protective effects of low-level laser therapy on bone loss to provide new insight into its therapeutic utility for postmenopausal osteoporosis. Experimental procedure: 24 female rats were randomly divided into 4 groups: sham operation (sham), ovariectomy (OVX), ovariectomy with needle acupuncture (O-N), and ovariectomy with laser acupuncture (O-L). O-N received needle acupuncture on the acupoints of Shenshu (BL23), Housanli (ST36) and Xuanzhong (GB39), whereas O-L received laser acupuncture on the same points. After the treatment period ended, the rats were euthanized to evaluate bone mass, microstructure, by micro-CT and histological staining. The transcription of bone formation-related genes by RT-PCR. Markers of bone metabolism was determined in serum were quantified by ELISA. Results and conclusion: O-L induced significant improvements in bone density and trabecular microarchitecture, showing significant increase in BMD, Tb.Th, Tb.N, BV/TV and Conn.D, as well as significant decline in Tb.Sp and SMI. The results mirrored the findings obtained from histological examination. Moreover, O-N versus O-L, Col-1 in O-L significant increase (P<0.05). Both forms of acupuncture treatment significantly stimulated the transcription of ALP, BMP, OCN, Col-1, RUNX- 2 and OPG. Laser acupuncture can mitigate bone loss, stimulate osteogenesis, and repair damaged trabecular network in ovariectomized rats. These results provided preliminary evidence that laser acupuncture could be applied as a non-invasive method to osteoporosis treatment.
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Jianhua Xu, Bitan Zheng
This work aimed to explore the clinical efficacy of orthodontic combined with orthognathic treatment in patients with skeletal class III malocclusion and mandibular deviation. This work compared the occlusal function, soft tissue symmetry, treatment satisfaction, numerical rating scale (NRS), and Holdaway measures of 60 patients before and after treatment. The results showed that after treatment, the surface recruitment potential of different muscle bundles in the patient's face increased, which was more obvious at 6 months (P < 0.05). The facial muscle asymmetry of the patients all increased and decreased greatly after 3 and 6 months of treatment, respectively. After 3 months and 6 months of treatment, the deviation of the upper lip and the lower lip on both sides of the patient decreased, and the deviation of the mandible on both sides increased (P < 0.05). The patients' satisfaction with orthodontic combined with orthognathic treatment reached 96.67%, and the complication rate was 23.33%. After treatment, the NRS scores of anteroposterior radiographs in different parts were increased, the NRS scores of the lateral, inferior 1/3, and inferior 2/3 parts of the face were increased, while the NRS scores of the upper 1/3 and mid-face 1/3 parts were decreased. After treatment, the patient's A point convexity, Sn-H line, H angle, and mentiolabial groove depth were observably increased, while Li-H line, upper labial groove depth, upper lip thickness, chin thickness, and nasal convexity were sharply decreased (P < 0.05). In conclusion, orthodontic combined with orthognathic treatment can improve the occlusal function and soft tissue asymmetry of patients effectively, which was beneficial to the recovery of facial function and improved the facial aesthetics of patients.
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Ying Dong, Guiyue Wang, Mengxue Zhang, Yiqing Yin
Objective: Based on clinical data, to study the effect of different anesthetic drugs on pharmacological modulation of postoperative pain relief in oncology patients. Methods One hundred twenty-six oncology patients who underwent surgery in a tertiary hospital from January 2020 to January 2024 were retrospectively selected as study subjects, and three anesthetic analgesic regimens of opioid analgesia, local anesthetic analgesia, and combined analgesia were used to record the pain scores (VAS scores) at 30min, 1h, 3h, 6h, 12h, 24h, and 48h postoperatively, as well as the patient's 24h postoperatively The number of self-controlled analgesia (PCIA) presses and analgesic remedy rate were recorded. Results The VAS scores of the composite analgesia group were lower than those of the other two groups at all postoperative time points, and the differences were statistically significant from 30 min to 6 h postoperatively (P<0.05). There were significant differences in the number of PCIA presses and analgesic remedy rates among the three groups in the 24h postoperative period (F=7.45, 8.92, P=0.001, 0.000). The analgesic remedy rate of 15.2% in the compound analgesia group was significantly lower than that of 28.6% in the opioid analgesia group and 34.9% in the local anesthetic analgesia group, P<0.05. Conclusion Based on the analysis of clinical data, the composite analgesic regimen can enhance the analgesic effect while reducing the use of opioids, lowering the risk of related adverse reactions, and improving patients' postoperative comfort.
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Youshen Cao, Yaonan Li, Sicheng Xiao
Gut microbiota are the microorganisms living in the human intestine, play a pivotal role in synthesizing of various substances essential for growth, development and overall body’s health. Exercise physiology has its effects on skeletal muscle bioenergetics, endocrine signaling, and systemic metabolic adaptation which directly and indirectly regulates the gut microbial ecosystem. There is a bidirectional interaction between physical exercise and gut microbiota which exerts a significant impact on human metabolic health and gastrointestinal function. By reviewing relevant domestic and international literature, this article summarizes the relationships among exercise, gut microbiota, metabolic health, and gastrointestinal function insight of biological sciences. Firstly, it introduces the regulatory effect of exercise on gut microbiota and its diversity, clarifies the impact of different exercise types on the structure of gut microbiota, and explains the potential mechanisms of exercise regulating gut microbiota. Secondly, it presents multiple mechanisms through which gut microbiota exert reciprocal effects on exercise-related health. Finally, it elucidates the influence of the "bidirectional regulatory" network between exercise and gut microbiota on metabolic health and gastrointestinal function, providing a theoretical basis for the prevention and treatment of metabolic diseases and gastrointestinal dysfunction.
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Xianjing Yu, Zhongcui Lu, Maosen Feng
Objective: To investigate the clinical and pathological characteristics of patients with gastric precancerous lesions and Helicobacter pylori (HP) infection, and to analyze the relationship between HP infection and SEMA5A gene polymorphism. Methods: A total of 70 patients with gastric precancerous lesions admitted to our hospital from January 2024 to January 2025 were randomly selected as the research subjects. According to their HP infection status, HP-infected patients were included in the infection group. Patients without HP infection were included in the non-infection group. The clinical and pathological characteristics of the two groups were compared. Whole blood specimens of all patients were collected and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) was used to detect SEMA5A genotypes, and the relationship between HP infection and SEMA5A genotype distribution in patients with gastric precancerous lesions was analyzed. Results: The proportion of lesion sites between the two groups was significantly different (P<0.05). Among them, the proportion of gastric antrum and gastric angle in the infection group was high, while the proportion of cardia and gastric body in the non-infection group was high. The proportion of ulcers in the infection group was higher than that in the non-infection group (P<0.05). There was no significant difference in age, gender, and color ratio between the two groups (P>0.05). The SEMA5A genotype distribution between the two groups was significantly different (P<0.05). The SEMA5A gene in the infected group was mainly TT genotype, accounting for 53.33%, which was higher than that in the uninfected group. Conclusion: The lesions of patients with gastric precancerous lesions and HP infection are mostly distributed in the gastric antrum and gastric angle, and the mucosal surface is often accompanied by ulcers. HP infection in patients with gastric precancerous lesions is closely related to SEMA5A gene polymorphism.
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Lingli Bai, Na Yang
To explore the influencing factors of pregnancy complications in older parturients and the construction and efficacy verification of prediction models. Methods: A total of 141 older parturients from June 2023 to January 2025 were selected as the subjects and divided into an occurrence group and a non-occurrence group according to whether complications occurred during pregnancy. The types of pregnancy complications of the patients were counted, and the medical records of the two groups were reviewed. The possible influencing factors of pregnancy complications in older parturients were analyzed by univariate and multivariate analysis. R software was used to construct a prediction model for the occurrence of pregnancy complications in older parturients, and the prediction efficacy was verified. Results: A total of 42 older parturients had pregnancy complications (accounting for 29.79%). The top three were anemia, hypertensive disorders complicating pregnancy, and gestational diabetes. The results of univariate and multivariate Logistic regression analysis showed that age, number of prenatal examinations, whether there was excessive nutrient intake during pregnancy, bad living habits, psychological pressure, and mood swings were risk factors for complications during pregnancy in older mothers (P<0.05). A prediction model for complications during pregnancy in older mothers was successfully constructed, and the slope of the calibration curve was high (close to 1), and the curve area was 0.817 (95%CI was 0.759-0.871). The calibration and standard curves had a good fit and good consistency. Conclusion: The incidence of complications during pregnancy in older mothers is high, and there are many influencing factors. Based on the multivariate results, a prediction model for complications during pregnancy in older mothers was successfully constructed, which has high prediction sensitivity, specificity, discrimination, and calibration.
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Liguo Qiu, Xionghui Guo, Jian Gong, Mingqiang Liu, Fengming Hu
This article reports a case of a child with mucous eccrine nevus on the waist. The patient had brown plaques on the left lumbar spine, accompanied by sweating symptoms, and the plaques were formed 4 years and 2 months ago. However, since the patient did not experience itching or pain when the disease occurred, no attention was paid and no treatment was given, and it was believed to be related to exercise and friction. After dermatological examination, skin CT and pathological examination of lesion tissue, the child was finally diagnosed with mucous eccrine nevus, and the lesion was brown. Symptomatic supportive interventions such as observation and waiting, drug therapy, physical therapy and surgical treatment were given to the child, all of which achieved good treatment effects. Due to the special location of the case and the clear diagnosis of the patient, after discussion with the child and his parents, no treatment was recommended, and during the 3-year follow-up, the skin lesions did not disappear, and the area and nature of the skin lesions did not change. However, the pathogenic MEN site of this child is the first report in the world, which has high clinical academic value and can provide a basis and reference for the diagnosis and treatment of similar cases.
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Xinran Zhou, Chunrong Hu, Haicheng Zhou, Ruixuan Yu, Shuyue Zhang, Bowen Zheng
Pasteurella multocida is a rare human pathogen and cases of infection caused by this pathogen following a tiger bite are extremely rare. Here, we describe the case of a patient in our hospital with an infection of the bloodstream caused by P. multocida following a tiger bite. We discuss and analyze the case and provide the best treatment plan according to the patient's manifestations and clinical symptoms.
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Pengwei Zhu
This research project aims to explore and understand the mechanisms associated with the pathogenesis of BD through specific aims: Develop a standard analytic pipeline for preprocessing and analyzing BCNs using rs-fMRI data and clinical and demographic records from the Adolescent Brain Cognitive Development (ABCD) study. This will allow us to quantitatively understand the cognitive development of adolescents with BD, ultimately improving diagnosis and treatment for this population. The goal of this research project is to address critical issues in the preprocessing and analysis of rs-fMRI data to enhance our comprehension of BD, develop more accurate methods for diagnosis and treatment, and reduce the public health impact of this disorder.
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Zhenghui Huang, Junyi Zheng, Shubin Guo
Background: The relationship between depression and sepsis is interconnected, but the nature of their association remains unclear. Our aim is to determine whether the genetic susceptibility to depression is related to sepsis and 28-day mortality from sepsis. Methods: In the Mendelian randomization (MR) study, we accessed exposure data from Genome-wide association study (GWAS) and the FinnGen consortium. We employed univariable and reverse MR analyses to explore potential associations between depression and sepsis and its 28-day mortality. The causal estimates and direction between depression and sepsis were evaluated employing a two sample bidirectional MR strategy. The inverse variance weighted (IVW) method was the primary approach utilized. Various sensitivity analyses were performed to confirm the validity of the causal effect. Causality was inferred between correlated traits by Mendelian Randomization analyses. Results: Bidirectional Mendelian randomization analyses demonstrated no evidence of causal associations: genetically predicted depression showed no significant effect on sepsis risk (IVW OR=1.102, 95%CI=0.951-1.277, p=0.197), while genetic liability to sepsis was not associated with depression risk (IVW OR=0.992, 95%CI=0.937-1.051, p=0.786). Sensitivity analyses using weighted median and MR-Egger methods revealed consistent null effects with <5% variation in effect estimates. Furthermore, genetically instrumented depression exhibited no causal impact on 28-day sepsis mortality (IVW OR=1.051, 95%CI=0.746-1.482, p=0.775), with all confidence intervals encompassing unity (OR=1). Conclusions: In summary, this bidirectional Mendelian randomization study provides robust evidence that genetic susceptibility to depression is not causally associated with the risk of sepsis or 28-day mortality from sepsis. Similarly, reverse MR analysis revealed no significant causal effect of sepsis on the risk of depression. These findings challenge the hypothesis of a direct genetic link between depression and sepsis, suggesting that the observed epidemiological associations may be driven by shared environmental factors, inflammatory pathways, or other confounding variables rather than a direct causal relationship. The lack of a genetic causal association highlights the importance of exploring alternative mechanisms, such as immune dysregulation, metabolic disturbances, or psychosocial factors, that may underlie the complex interplay between mental health and critical illness outcomes. Future research should focus on identifying potential mediators, including inflammatory biomarkers (e.g., IL-6, TNF-α) and metabolic pathways, to better understand the pathophysiology of sepsis and its long-term psychological sequelae. Despite the negative findings, this study contributes to the growing body of literature on the relationship between mental health and sepsis by employing a rigorous MR design that minimizes confounding and reverse causality. The results underscore the need for further investigation into the role of non-genetic factors in the development of sepsis and its outcomes, as well as the potential for targeted interventions to improve the mental and physical health of sepsis survivors.
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Haibin Lu, Chen Liang
Objective: To investigate the therapeutic effects of tetrandrine and arsenic trioxide (ATO) on breast cancer using a nude mouse xenograft model. Methods: A breast cancer xenograft model was established in nude mice using the MDA-MB-435S cell line. The mice were divided into treatment groups as follows: Tet group (tetrandrine), ATO group (arsenic trioxide), Tet+ATO group (combination therapy), CDDP group (cisplatin), and CON group (normal saline control). After 4 weeks of treatment, the therapeutic effects and potential mechanisms of the different drugs were evaluated through gross observation, histological examination, and immunohistochemical analysis of tumor and lung tissues. Results: Compared with the control group, tumor volume and mass were significantly reduced in the CDDP, Tet, ATO, and Tet+ATO groups (P < 0.05). The Tet+ATO group showed significantly greater reduction in tumor volume and mass than the Tet or ATO alone groups, though these values were higher than those in the CDDP group (P < 0.05). Gross examination revealed numerous gray-white nodules in the lungs of all control animals. Fewer nodules were observed in the Tet (4/8) and ATO (5/8) groups, while the CDDP (1/8) and Tet+ATO (3/8) groups exhibited markedly fewer nodules than the CON, Tet, and ATO groups. Immunohistochemistry showed significantly decreased MMP2 expression in the tumors of all drug-treated groups compared with the control, while MMP9 expression was increased primarily in fibrotic and granulation tissue areas. VEGF and CD31 expression were significantly lower in the CDDP and Tet+ATO groups than in the control. Conclusion: Tetrandrine, arsenic trioxide injection, and their combination all inhibited tumor growth and metastasis. The combined use of tetrandrine and arsenic trioxide reduced the required dosage of arsenic trioxide without compromising its inhibitory effect on breast cancer growth, while also decreasing the incidence of lung metastasis. The antitumor mechanism of the combination therapy is associated with the regulation of tumor angiogenesis and extracellular matrix remodeling.
